Why Advanced LCMS
Your 99% Pure Peptide Isn't.
Standard purity certificates report what UV light absorbs. Mass spectrometry reveals what your peptide actually is.
99.63%
UV-Vis Reported
94.38%
LC-MS Actual
5.25 percentage points of unknown substances — invisible to standard testing
The Problem
What 5% Actually Means
A 5% purity gap could be harmless structural variants or counter-ion salts — or it could be harmful levels of degradation products and synthesis by-products. UV-Vis cannot tell the difference. LC-MS can.
55
Contaminants
Detectable by LC-MS
Types of impurities we screen for:
Degradation & Structural Variants
Oxidation, deamidation, hydrolysis — affect efficacy, rarely an immediate health risk
Synthesis Process Residues
TFA salts, Fmoc, tBu — accumulate with repeated dosing, long-term concern
Hazardous Adulterants
Fentanyl analogues, unknown pharmacologicals — immediate health risk, flagged critical
Actual risk is determined by the detected quantity of each specific compound relative to established safety thresholds — not by impurity type alone.
Standard Testing
The Blind Spot in UV-Vis Testing
UV-Vis measures how much light a sample absorbs. It does not reveal molecular identity. A peptide fragment with similar absorption properties is completely invisible to UV detection — it reads as "pure" peptide. Structural alterations, synthesis residues, and dangerous adulterants routinely pass UV-Vis undetected.
- ·Oxidized peptide variants (+16 Da)
- ·Deamidated sequences (+1 Da)
- ·Residual TFA, Fmoc, tBu from synthesis
- ·Hydrolyzed or cleaved fragments
- ·Structural alterations and isomers
- ·Exact molecular mass (±0.01 Da)
- ·Every modification & structural variant
- ·Hidden impurities by precise mass shift
- ·Sequence integrity via fragmentation
- ·Structural fingerprint analysis
Impurity Classification
Not All Impurities Are Equal
We classify every detected impurity by its health risk profile — so you know exactly which findings require action and which are informational.
Category 1
Degradation & Variants
Risk depends on detected concentration. Low levels are primarily an efficacy concern; elevated concentrations above established thresholds trigger a risk flag.
Category 2
Synthesis Residues
Any detection above trace levels is flagged. These residues accumulate with repeated dosing and present a long-term concern at elevated concentrations.
Category 3
Hazardous Adulterants
Any detection — regardless of quantity — triggers a critical alert. No safe threshold exists for pharmacological adulterants.
Complete Analysis
What Our Report Covers
Four analytical pillars that give you the complete picture of your peptide's quality and safety — in a single report.
UV & MS Purity
Both methods, side by side
We report UV-Vis purity and MS-based purity separately. UV and MS don't always agree — and when they don't, the difference tells you exactly what's hiding in your sample.
Impurity Identity & Quantity
Named, measured, explained
Every impurity is identified by exact mass shift and quantified as a percentage. You see what the impurities are, not just that they exist — oxidation products, deamidation, hydrolysis fragments, each named and measured.
Contamination Detection
Synthesis residuals & hazardous adulterants
Screens for peptide synthesis process residuals (TFA, Fmoc, tBu, coupling reagents) and over 23 hazardous adulterant compounds. Any detection is explicitly reported — present or absent, no ambiguity.
Molecular Identity
High-res mass confirmation
High-resolution mass spectrometry confirms the correct peptide to sub-ppm accuracy. MS/MS fragmentation provides sequence coverage and structural fingerprint similarity — three independent layers that counterfeits cannot pass.
Potency & Content
Actual peptide mass vs. label claim
Is your "10 mg" vial actually 10 mg? We quantify exact peptide content using certified reference standards (CRMs). Underdosing is common in grey-market supply — this tells you precisely what you're working with.
Clear Results
The Scoring System
Complex analytical data distilled into one actionable number you can understand at a glance — and act on immediately.
Overall Score
A single number (1–10) combining purity, potency, and integrity metrics, weighted by clinical importance.
Rating
Excellent, Good, Warning, or Poor — instant quality assessment.
Risk Flags
Specific hazards highlighted: Alteration Risk and Impurities Hazard levels with colour-coded indicators.
What Triggers a Warning
Oxidation >3%, methylation >1.5%, or deamidation >0.2% — indicates degraded or improperly stored peptide.
Hydrolysis >1%, cleavage >0.7%, or synthesis residues (TFA, Fmoc, tBu) above safety thresholds.
Lab Infrastructure
Research-Grade Equipment
Our partner facility uses instruments designed for pharmaceutical research — not just QC screening. The same class of equipment used by major pharma corporations.
Agilent 6520 LC/Q-TOF
Primary platform. >30,000 resolution, <0.8 ppm mass accuracy. ESI, APCI, and ASAP sources. Gold standard for peptide identity and impurity profiling.
Agilent 6520 Q-TOF
Secondary high-resolution system. Confirmatory analysis and method redundancy, coupled to Agilent 1200 HPLC with DAD.
Agilent 6420 Triple Quad
Targeted MS/MS quantification. High selectivity and exceptional sensitivity for trace-level contaminant detection and confirmation.
Agilent 1260 HPLC / DAD
Routine chromatographic quantification and UV purity assays. Multi-wavelength detection ensures no impurity hides under the main peak.
Independent
We don't sell peptides. Zero conflict of interest. Our only product is accurate data.
Academic Rigor
PhD-level analytical chemists. Research-grade validated workflows. Not a QC rubber stamp.
Verified Reports
Every report has a unique ID and QR code. Tamper-proof, publicly verifiable — share it with confidence.
The Bottom Line
Why This Matters
A vendor's UV-Vis certificate might look perfect...
...but MS reveals significant impurities invisible to UV.
We flag it so you can make an informed decision.